APPLICATION OF FRIT FAST-ATOM-BOMBARDMENT LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY FOR THE DETERMINATION OF ACETYLCHOLINE LEVELS IN RAT-BRAIN REGIONS

被引：24

作者：

IKARASHI, Y ^{[1
]}

ITOH, K ^{[1
]}

MARUYAMA, Y ^{[1
]}

机构：

[1] GUNMA UNIV,SCH MED,DEPT NEUROPSYCHOPHARMACOL TSUMURA,3-39-22 SHOWA MACHI,MAEBASHI,GUNMA 371,JAPAN

来源：

BIOLOGICAL MASS SPECTROMETRY | 1991年 / 20卷 / 01期

关键词：

D O I：

10.1002/bms.1200200106

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

This report describes the use of FRIT fast atom bombardment (FAB) liquid chromatography/mass spectrometry for the analysis of acetylcholine in rat brain regions. Direct assessment of acetylcholine levels is possible without the need for either derivatization or extensive sample preparation. Quantification is accomplished by monitoring intact molecular cations of acetylcholine and a deuterated internal standard. The results are compared with those obtained by conventional pyrolysis gas chromatography/mass spectrometry and by liquid chromatography with electrochemical detection.

引用

页码：21 / 25

页数：5

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