EFFECTS OF CHRONIC EXPOSURE OF NG108-15 CELLS TO MORPHINE OR ETHANOL ON BINDING OF NUCLEAR FACTORS TO CAMP-RESPONSE ELEMENT

被引：17

作者：

OSUGI, T ^{[1
]}

TANIURA, H ^{[1
]}

IKEMOTO, M ^{[1
]}

MIKI, N ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT PHARMACOL 1,KITA KU,OSAKA 530,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90479-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gel retardation assay with a single-stranded oligo-DNA of cAMP-response element (CRE) in a somatostatin promoter region was selected to examine the possibility of transcriptional regulation of cAMP-inducible genes by chronic morphine or ethanol treatment of NG108-15 cells. When the nuclear extracts from the cells treated with morphine (50 μM) or ethanol (100 mM) for several days were assayed, the amount of DNA-protein comples was decreased about 30-40% compared to that of the control. The decreased complex was recovered by 1-2 days after withdrawal of the drugs. Treatment of the cells with these drugs for 1 h did not change the amount of the DNA-protein complex. Thus, changes in CRE-binding proteins from the cells treated chronically with morphine or ethanol suggest that these drugs can modulate the expression of cAMP-inducible genes through which tolerance and dependence may develop. © 1991.

引用

页码：25 / 31

页数：7

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