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MURINE RECOMBINANT LEUKEMIA INHIBITORY FACTOR MODULATES INHIBITORY EFFECT OF 1,25-DIHYDROXYVITAMIN-D3 ON ALKALINE-PHOSPHATASE ACTIVITY IN MC3T3-E1 CELLS

被引：9
作者：
HAKEDA, Y
SUDO, T
ISHIZUKA, S
TANAKA, K
HIGASHINO, K
KUSUDA, M
KODAMA, H
KUMEGAWA, M
机构：
[1] MEIKAI UNIV,SCH DENT,DEPT ORAL ANAT,SAITAMA 35002,JAPAN
[2] BIOMAT INST,YOKOHAMA 244,JAPAN
[3] OHU UNIV,SCH DENT,DEPT ORAL ANAT,KORIYAMA 963,JAPAN
[4] TEIJIN INST BIOMED RES,HINO,TOKYO 191,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 02期
关键词：
D O I：
10.1016/0006-291X(91)91604-B
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We demonstrated murine leukemia inhibitory factor (mLIF) mRNA in osteoblastic MC3T3-E1 cells, but not mLIF in their conditioned medium. Recombinant mLIF had an inhibitory effect on alkaline phosphatase (ALP) activity, but not on DNA synthesis, in these mLIF-free cells. This inhibitory effect was not prostaglandin E2 dependent. mLIF also modulated the inhibitory effect of 1,25 dihydroxyvitamin D3 [1,25(OH)2D3] on ALP activity, partly via down regulation of 1,25(OH)2D3 binding sites. These results suggest that LIF may play a role in regulating osteoblast differentiation. © 1991.
引用
收藏
页码：577 / 582
页数：6
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