VIBRIOPHAGE-D10 CONTAINS NON-PERMUTED DNA WITH COHESIVE ENDS

Phage D10. a Vibrio cholerae O-1 El Tor group X phage, is one of the five newly isolated phages used in the phage typing scheme developed for V. cholerae O-1 biotype E1 Tor and belongs to the Myoviridae family. From electron microscopic studies it is shown that phage DIO has a DNA genome of 32 +/- 0.2 kb. This is the first report where it has been shown by the construction of a partial denaturation map that this vibriophage genome is non-permuted and has cohesive ends. The location of the ends of the DNA in the phage head has also been inferred.