PURIFICATION AND CHARACTERIZATION OF AN OXYGEN-LABILE, NAD-DEPENDENT ALCOHOL-DEHYDROGENASE FROM DESULFOVIBRIO-GIGAS

被引:39
作者
HENSGENS, CMH
VONCK, J
VANBEEUMEN, J
VANBRUGGEN, EFJ
HANSEN, TA
机构
[1] UNIV GRONINGEN,DEPT MICROBIOL,KERKLAAN 30,9751 NN HAREN,NETHERLANDS
[2] UNIV GRONINGEN,BIOSON RES INST,9747 AG GRONINGEN,NETHERLANDS
[3] STATE UNIV GHENT,DEPT BIOCHEM PHYSIOL & MICROBIOL,B-9000 GHENT,BELGIUM
关键词
D O I
10.1128/JB.175.10.2859-2863.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A NAD-dependent, oxygen-labile alcohol dehydrogenase was purified from Desulfovibrio gigas. It was decameric, with subunits of M(r) 43,000. The best substrates were ethanol (K(m), 0.15 mM) and 1-propanol (K(m), 0.28 mM). N-terminal amino acid sequence analysis showed that the enzyme belongs to the same family of alcohol dehydrogenases as Zymomonas mobilis ADH2 and Bacillus methanolicus MDH.
引用
收藏
页码:2859 / 2863
页数:5
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