ITS2 ribosomal RNA indicates Schistosoma hippopotami is a distinct species

被引:42
作者
Despres, L
Kruger, FJ
ImbertEstablet, D
Adamson, ML
机构
[1] S AFRICAN MRC,NELSPRUIT 1200,SOUTH AFRICA
[2] UNIV PERPIGNAN,CTR BIOL & ECOL MEDITERRANEENNE & TROP,F-66860 PERPIGNAN,FRANCE
[3] UNIV BRITISH COLUMBIA,DEPT ZOOL,VANCOUVER,BC V6T 1Z4,CANADA
基金
加拿大自然科学与工程研究理事会;
关键词
Schistosoma hippopotami; species status; ITS2 rRNA sequences;
D O I
10.1016/0020-7519(95)00048-8
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The internal transcribed spacer region of the ribosomal RNA, ITS2, was sequenced from a single specimen of S. hippopotami collected from a pulmonary artery of the hippopotamus, Hippopotamus amphibius in South Africa. The nucleotide sequence was aligned with those of S. mansoni S. rodhaini, S. haematobium, S. intercalatum, S. curassoni, S. bovis and S. japonicum. Both maximum parsimony and genetic distance analyses were performed on these data sets. Using S. japonicum as outgroup to the African schistosomes, a single most-parsimonious tree was obtained of length 64 steps with a consistency index of 1. S. hippopotami was the sister-group to the remaining African species. This species has lateral-spined eggs and its basal position in the tree suggests that this condition is primitive and that terminal-spined eggs developed secondarily. Molecular data clearly show that S. hippopotami cannot be considered synonymous with S. mansoni. Assuming the hippopotamus is the normal host of S. hippopotami, phylogenetic analysis is consistent with an ancient association between schistosomes and ungulates.
引用
收藏
页码:1509 / 1514
页数:6
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