MOLECULAR-CLONING AND TISSUE EXPRESSION OF HUMAN MITOCHONDRIAL 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE

被引：42

作者：

MASCARO, C ^{[1
]}

BUESA, C ^{[1
]}

ORTIZ, JA ^{[1
]}

HARE, D ^{[1
]}

HEGARDT, FG ^{[1
]}

机构：

[1] UNIV BARCELONA,FAC FARM,SCH PHARM,BIOCHEM UNIT,E-08028 BARCELONA,SPAIN

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1995年 / 317卷 / 02期

关键词：

D O I：

10.1006/abbi.1995.1178

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitochondrial 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase is a constituent of the HMG-CoA pathway responsible for ketone body synthesis and one of its main regulatory points. We report the isolation and characterization of a 2058-bp cDNA from human liver. This cDNA encodes a polypeptide of 508 residues and 56,635 Da. The homology with previously reported rat mitochondrial and human cytosolic HMG-CoA synthases is 88 and 66%, respectively. mRNA levels were high in liver and colon, low in testis, heart, skeletal muscle, and kidney, and faint in pancreas. (C) 1995 Academic Press, Inc.

引用

页码：385 / 390

页数：6

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