Biochemical evidence for the presence of a bifunctional dihydrofolate reductase-thymidylate synthase in plant species

被引：5

作者：

Balestrazzi, A ^{[1
]}

Branzoni, M ^{[1
]}

Carbonera, D ^{[1
]}

Parisi, B ^{[1
]}

Cella, R ^{[1
]}

机构：

[1] UNIV PAVIA, DIPARTIMENTO GENET & MICROBIOL A BUZZATI TRAVERSO, I-27100 PAVIA, ITALY

来源：

JOURNAL OF PLANT PHYSIOLOGY | 1995年 / 147卷 / 02期

关键词：

Daucus carota; dihydrofolate reductase-thymidylate synthase; Nicotiana plumbaginifolia (Doba cell line); Oryza sativa; isozymes;

D O I：

10.1016/S0176-1617(11)81515-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

It is shown that wild carrot cells contain both a bifunctional DHFR-TS (EC 2.1.1.45)land a monofunctional DHFR (EC 1.5.1.3). The bifunctional protein was present as the major DHFR fraction (80%) eluted from MTX-Sepharose. SDS-PAGE and immunoblot experiments showed that wild and domesticated carrot DHFR-TS have the same molecular mass (about 58 kDa). The immunochemical analysis showed the occurrence of the 58 kDa polypeptide also in the Nicotiana plumbaginifolia MTX-Sepharose fraction, whereas a band of about 50 kDa was recognized in Oryza sativa. DHFR and TS activities were present in both purified fractions. These data indicate that the bifunctional DHFR-TS is ubiquitous in plant cells.

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收藏

页码：263 / 266

页数：4

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