STRUCTURE OF A RHODOCOCCUS GENE ENCODING PIGMENT PRODUCTION IN ESCHERICHIA-COLI

被引:19
作者
HART, S [1 ]
KIRBY, R [1 ]
WOODS, DR [1 ]
机构
[1] UNIV CAPE TOWN,DEPT MICROBIOL,RONDEBOSCH 7700,SOUTH AFRICA
来源
JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷
关键词
D O I
10.1099/00221287-136-7-1357
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A 2.1 kbp DNA fragment from Rhodococcus strain ATCC 21145 gave rise to the production of blue and pink pigments in Escherichia coli when cloned downstream of a strong promoter. The sequence of this DNA fragment contains a single open reading frame with a putative ribosome-binding site, potentially coding for a single protein of M(r) 42,560. Deletion analysis and in vitro transcription-translation experiments support the hypothesis that pigment production in E. coli is due to a single enzyme whose catalytic activity is still unknown. This small pigment gene may become useful for the development of a new generation of chromogenic cloning vectors which do not require expensive substrates for the detection of gene expression.
引用
收藏
页码:1357 / 1363
页数:7
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