PRESENCE OF POLY(A) IN A FLAVIVIRUS - SIGNIFICANT DIFFERENCES BETWEEN THE 3' NONCODING REGIONS OF THE GENOMIC RNAS OF TICK-BORNE ENCEPHALITIS-VIRUS STRAINS

A poly(A) tail was identified on the 3' end of the prototype tick-borne encephalitis (TBE) virus strain Neudoerfl. This is in contrast to the general lack of poly(A) in the genomic RNAs of mosquito-borne flaviviruses analyzed so far. Analysis of several closely related strains of TBE virus, however, revealed the existence of two different types of 3' noncoding (NC) regions. One type (represented by strain Neudoerfl) is only 114 nucleotides long and carries a 3'-terminal poly(A) structure. This was also found in several TBE virus strains isolated from different geographic regions over a period of almost 30 years. The other type (represented by strain Hypr) is 461 nucleotides long and not polyadenylated. The sequence homology between the two types of TBE virus 3' NC regions terminates at a specific position 81 nucleotides after the stop condon. The second type of 3' NC region more closely resembles the common flavivirus pattern, including the potential for the formation of a 3' -terminal hairpin structure. However, it lacks primary sequence elements that are conserved among other flavivirus genomes.