HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF A POTENTIAL MEMORY-ENHANCING COMPOUND (CL-275,838) AND ITS DESBENZYL METABOLITE IN RAT PLASMA OR SERUM

被引：7

作者：

GUISO, G ^{[1
]}

CONFALONIERI, S ^{[1
]}

CACCIA, S ^{[1
]}

机构：

[1] MARIO NEGRI INST PHARMACOL RES,VIA ERITREA 62,I-20157 MILAN,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 572卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80502-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid, selective, precise reversed-phase liquid chromatographic method has been developed for the determination of a potential memory-enhancing agent (CL 275,838) and its main metabolite (CL 286,527) in plasma and serum. The procedure includes isolation of compounds from proteins precipitated with acetonitrile, subsequent resolution by reversed-phase (Whatman Partisphere C-8) high-performance liquid chromatography and ultraviolet detection. The assay was linear over the range 0.12-1.25-mu-g/ml of plasma or serum. The detection limit was 0.12-mu-g/ml, using 0.2 ml of plasma or serum. Intra- and inter-day validation studies indicated an acceptable precision and reproducibility of the method within the concentration range investigated, the overall coefficient of variation being less than 10%. The method is currently applied in support of pharmacological and toxicity studies of the compound in rodents.

引用

页码：346 / 351

页数：6

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