MICRODETERMINATION OF 2-DEOXYGLUCOSE AND 2-DEOXYGLUCOSE 6-PHOSPHATE TO DETERMINE GLUCOSE-UTILIZATION RATES IN SINGLE NEURONS AND SMALL CNS REGIONS AFTER INJECTING NONTRACER AMOUNTS OF 2-DEOXYGLUCOSE

被引:8
作者
AKABAYASHI, A [1 ]
KATO, T [1 ]
机构
[1] YOKOHAMA CITY UNIV,SCH MED,DEPT BIOCHEM,FUKUURA,KANAZAWAKU,YOKOHAMA 236,JAPAN
关键词
DEOXYGLUCOSE; GLUCOSE UTILIZATION; NEURON; ENZYMATIC MICROASSAY;
D O I
10.1111/j.1471-4159.1992.tb08327.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A nontracer amount (0.25 mmol/kg of body weight) of 2-deoxyglucose (DG) was intravenously injected into rats, which were frozen 2 and 4 min later in liquid nitrogen. Freeze-dried samples of CNS regions and cell bodies of spinal motor neurons were prepared, and the concentrations of glucose, glucose 6-phosphate, DG, and DG 6-phosphate (DG6P) in them were microassayed after 3,000-1,500,000-fold amplification using an enzymatic amplification reaction, NADP cycling. Based on the time course of glucose, DG, and DG6P concentrations in arterial plasma and the anterior horn of the spinal cord, the Soko-loff-type rate equations for DG and DG6P concentrations were mathematically solved, and the resultant DG and DG6P concentration functions were fitted to the data points using the nonlinear least-squares fitting SALS package program. This fitting provided four rate constants for the functions and supported the theoretical basis for our calculations of glucose utilization rate (GUR) when DG was administered in nontracer amounts. The GUR was highest in the spinal motor neurons and lowest in the white matter of the cerebellum. Neuron-rich structures, such as the cerebellar molecular and granular layers and the anterior horn of the spinal cord, had higher GUR values than the white matter of the cerebellum and spinal cord.
引用
收藏
页码:888 / 896
页数:9
相关论文
共 23 条
[1]   AN ENZYMATIC MICROASSAY METHOD FOR DEOXYGLUCOSE AND DEOXYGLUCOSE 6-PHOSPHATE [J].
AKABAYASHI, A ;
SAITO, T ;
KATO, T .
BIOMEDICAL RESEARCH-TOKYO, 1989, 10 (02) :173-177
[2]   ENZYMATIC ASSAYS FOR 2-DEOXYGLUCOSE AND 2-DEOXYGLUCOSE 6-PHOSPHATE [J].
CHI, MMY ;
PUSATERI, ME ;
CARTER, JG ;
NORRIS, BJ ;
MCDOUGAL, DB ;
LOWRY, OH .
ANALYTICAL BIOCHEMISTRY, 1987, 161 (02) :508-513
[3]   CEREBRAL GLUCOSE-UTILIZATION - COMPARISON OF [C-14] DEOXYGLUCOSE AND [6-C-14]GLUCOSE QUANTITATIVE AUTORADIOGRAPHY [J].
COLLINS, RC ;
MCCANDLESS, DW ;
WAGMAN, IL .
JOURNAL OF NEUROCHEMISTRY, 1987, 49 (05) :1564-1570
[4]   DOSE DEPENDENT REDUCTION OF GLUCOSE-UTILIZATION BY PENTOBARBITAL IN RAT-BRAIN [J].
CRANE, PD ;
BRAUN, LD ;
CORNFORD, EM ;
CREMER, JE ;
GLASS, JM ;
OLDENDORF, WH .
STROKE, 1978, 9 (01) :12-18
[5]   A METHOD FOR THE SIMULTANEOUS ESTIMATION OF REGIONAL RATES OF GLUCOSE INFLUX AND PHOSPHORYLATION IN RAT-BRAIN USING RADIOLABELED 2-DEOXYGLUCOSE [J].
CUNNINGHAM, VJ ;
CREMER, JE .
BRAIN RESEARCH, 1981, 221 (02) :319-330
[6]  
FROMM HJ, 1962, J BIOL CHEM, V237, P3027
[7]   BLOOD-BRAIN TRANSFER OF GLUCOSE AND GLUCOSE ANALOGS IN NEWBORN RATS [J].
FUGLSANG, A ;
LOMHOLT, M ;
GJEDDE, A .
JOURNAL OF NEUROCHEMISTRY, 1986, 46 (05) :1417-1428
[8]   STUDIES ON THE RELATIONSHIP BETWEEN CEREBRAL GLUCOSE-TRANSPORT AND PHOSPHORYLATION USING 2-DEOXYGLUCOSE [J].
HARGREAVES, RJ ;
PLANAS, AM ;
CREMER, JE ;
CUNNINGHAM, VJ .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1986, 6 (06) :708-716
[9]   MODELING THE DEPENDENCE OF HEXOSE DISTRIBUTION VOLUMES IN BRAIN ON PLASMA-GLUCOSE CONCENTRATION - IMPLICATIONS FOR ESTIMATION OF THE LOCAL 2-DEOXYGLUCOSE LUMPED CONSTANT [J].
HOLDEN, JE ;
MORI, K ;
DIENEL, GA ;
CRUZ, NF ;
NELSON, T ;
SOKOLOFF, L .
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1991, 11 (02) :171-182
[10]   DETERMINATION OF THE DEOXYGLUCOSE AND GLUCOSE PHOSPHORYLATION RATIO AND THE LUMPED CONSTANT IN RAT-BRAIN AND A TRANSPLANTABLE RAT GLIOMA [J].
KAPOOR, R ;
SPENCE, AM ;
MUZI, M ;
GRAHAM, MM ;
ABBOTT, GL ;
KROHN, KA .
JOURNAL OF NEUROCHEMISTRY, 1989, 53 (01) :37-44