INVITRO SELECTION OF OPTIMAL DNA SUBSTRATES FOR T4 RNA LIGASE

被引：14

作者：

HARADA, K

ORGEL, LE

机构：

[1] Salk Biological Studies Institute, P.O. Box 85800, San Diego

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 04期

关键词：

D O I：

10.1073/pnas.90.4.1576

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have used in vitro selection techniques to characterize DNA sequences that are ligated efficiently by T4 RNA ligase. We find that the ensemble of selected sequences ligated about 10 times as efficiently as the random mixture of sequences used as the input for selection. Surprisingly, the majority of the selected sequences approximated a well-defined consensus sequence.

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页码：1576 / 1579

页数：4

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