MEASUREMENT OF GLYCATED ALBUMIN BY THE NITROBLUE TETRAZOLIUM COLORIMETRIC METHOD

被引:30
作者
MASHIBA, S [1 ]
UCHIDA, K [1 ]
OKUDA, S [1 ]
TOMITA, S [1 ]
机构
[1] KYOTO HAKUAIKAI HOSP,KITA KU,KYOTO 603,JAPAN
关键词
NITROBLUE TETRAZOLIUM; ALBUMIN FRUCTOSAMINE; GLYCATED ALBUMIN; POLYETHYLENE GLYCOL;
D O I
10.1016/0009-8981(92)90133-B
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
A method has been developed for the measurement of glycated albumin (albumin-fructosamine) by the nitroblue tetrazolium (NBT) colorimetric method. In this method, polyethylene glycol was added to the serum and then the mixture was centrifuged to separate globulin proteins from albumin proteins. This made it possible to measure the glycated albumin in the supernatant by the NBT colorimetric method, without the interference of globulin proteins. This measurement method correlated with the measurement of glycated albumin using boronate affinity chromatography with an r value of 0.942 (P < 0.001). Our method using polyethylene glycol permits easy measurement of albumin fructosamine and is therefore useful as an index of diabetic control and for diabetic screening.
引用
收藏
页码:3 / 15
页数:13
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