DIRECT SEQUENCING OF SSP-PCR-AMPLIFIED CDNA TO IDENTIFY NEW ALLELES IN THE DR52-ASSOCIATED DRB1 GROUP - IDENTIFICATION OF DRB1-ASTERISK-1115, DRB1-ASTERISK-1117 AND DRB1-ASTERISK-1319

被引：20

作者：

ROBBINS, F

TANG, T

YAO, H

NG, J

HARTZMAN, RJ

HURLEY, CK

机构：

[1] GEORGETOWN UNIV, SCH MED, DEPT MICROBIOL & IMMUNOL, WASHINGTON, DC 20007 USA

[2] GEORGETOWN UNIV, SCH MED, DEPT PEDIAT, WASHINGTON, DC 20007 USA

[3] NATL NAVAL MED CTR, BETHESDA, MD 20814 USA

来源：

TISSUE ANTIGENS | 1995年 / 45卷 / 05期

关键词：

DIRECT DNA SEQUENCING; HLA; DR ALLELE; PCR WITH SEQUENCE SPECIFIC PRIMERS;

D O I：

10.1111/j.1399-0039.1995.tb02458.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Low and high resolution sequence specific oligonucleotide probe hybridization patterns were used to design an approach to direct sequencing of allele specific amplified cDNA. Several PCR amplifications were used to derive overlapping sequence fragments to define complete first domain sequences for a single allele. This method has been used to characterize three new DRB1 alleles in the DR52 family, DRB1*1115, DRB1*1117, and DRB1*1319. AU three alleles carry polymorphisms previously observed in other DRB alleles and underscore the importance of utilizing a directed sequencing approach for obtaining unambiguous typing results in matching for bone marrow transplantation between unrelated donor and recipient.

引用

页码：302 / 308

页数：7

共 17 条

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