A hypervariable middle repetitive DNA sequence from citrus

被引：17

作者：

Orford, SJ ^{[1
]}

Scott, NS ^{[1
]}

Timmis, JN ^{[1
]}

机构：

[1] CSIRO,DIV HORT,ADELAIDE,SA 5001,AUSTRALIA

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 91卷 / 08期

关键词：

DNA fingerprinting; citrus; hypervariable DNA; cultivar typing; somatic variation;

D O I：

10.1007/BF00220936

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The use of hypervariable sequences for DNA typing of plants is focussed on microsatellites and on amplification of regions defined by random (RAPD) or defined (AFLP) primers for PCR analysis of genomes. A hypervariable length of middle repetitive DNA has been isolated from citrus that contains no obvious hypervariable structures. The fingerprinting probe was shown to have an important commercial application in the separation of zygotic from nucellar progeny. A somatic variant of the sequence within one orange tree suggests that somatic variation in hypervariable markers may be a common event.

引用

页码：1248 / 1252

页数：5

共 27 条

[1]

Alexander, Some citrus species and varieties in Australia, (1983)

[2]

Cai Q., Guy C.L., Moore G.A., Extension of the linkage map in citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci, Theor Appl Genet, 89, pp. 606-624, (1994)

[3]

Cameron J.W., Frost H.B., Genetics, breeding and nucellar embryony, The citrus industry, vol. 2, pp. 325-370, (1968)

[4]

Cullis C.A., Sequence variation and stress, Genetic flux in plants, pp. 157-168, (1985)

[5]

Devereaux J., Haerberli P., Smithies O., A comprehensive set of sequence analysis programs for the VAX, Nucleic Acids Research, 12, pp. 387-395, (1984)

[6]

Frost H.B., Soost R.K., Seed reproduction: development of gametes and embryos, The citrus industry, vol. 2, pp. 290-324, (1968)

[7]

He M., Wilde A., Kaderbhai M.A., A simple single-step procedure for small-scale preparation of Escherichia coli plasmids, Nucleic Acids Res, 18, (1990)

[8]

Henikoff S., Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing, Gene, 28, pp. 351-359, (1984)

[9]

Hodgson R.W., Horticultural varieties of citrus, The citrus industry, vol. 1, pp. 431-591, (1967)

[10]

Jeffreys A.J., Wilson V., Thein S.L., Individual specific “fingerprints” of human DNA, Nature, 316, pp. 76-79, (1985)

← 1 2 3 →