CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST THE P17 CORE PROTEIN OF THE HUMAN IMMUNODEFICIENCY VIRUS-1

被引：8

作者：

COGNIAUX, J

DESCHEPPER, N

BLONDIAU, ML

CORNET, B

HORAL, P

VAHLNE, A

机构：

[1] UNIV LIBRE BRUXELLES,MICROMOLEC INTERFACES LAB,B-1050 BRUSSELS,BELGIUM

[2] GOTHENBURG UNIV,DEPT CLIN VIROL,S-41124 GOTHENBURG,SWEDEN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 128卷 / 02期

关键词：

ELISA; sandwich; HIV-1; Monoclonal antibody; p17 core protein;

D O I：

10.1016/0022-1759(90)90207-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Five mouse hybridomas which produce monoclonal antibodies against the p17 core protein of HIV-1 have been isolated. Cross-competition assays and mapping with synthetic peptides demonstrate that two closely related epitopes are identified by these antibodies. Directed against two neighbouring peptides at the carboxy-terminal end of the molecule, they can be used for the selective detection of p17 polypeptide in a viral extract or in an infected cell lysate by a solid-phase sandwich enzyme immunoassay. © 1990.

引用

页码：165 / 175

页数：11

共 29 条

[1]

BARRESINOUSSI F, 1983, SCIENCE, V220, P86

[2]

CHASSAGNE J, 1986, J IMMUNOL, V136, P1442

[3] PRODUCTION OF MONOCLONAL-ANTIBODIES AGAINST HTLV-I PROTEINS RECOGNIZING SURFACE EPITOPES OF LIVE INFECTED-CELLS [J].