CHARACTERIZATION OF STRAINS OF XANTHOMONAS-CAMPESTRIS PV HOLCICOLA BY PAGE OF MEMBRANE-PROTEINS AND BY REA AND RFLP ANALYSIS OF GENOMIC DNA

Polyacrylamide gel electrophoresis (PAGE) of membrane proteins, restriction endonuclease analysis (REA), and restriction fragment length polymorphism (RFLP) analysis of genomic DNA were used to characterize strains of Xanthomonas campestris pv. holcicola. After PAGE, a common membrane protein pattern was observed for 19 strains of X. c. pv. holcicola from Australia, Lesotho, Mexico, New Zealand, the Republic of South Africa, and the United States of America. This profile was distinct from the 22 other pathovars of X. campestris tested. Differences in fragmentation patterns were observed between strains of X. c. pv. holcicola after electrophoretic separation of EcoRl-digested genomic DNA in agarose gels (REA) or hybridization analysis after blotting the separated DNA to membranes (RFLP). However, DNA fragmentation patterns were distinguishable by both REA and RFLP, which were common to all strains of X. c. pv. holcicola but different from strains of other Xanthomonas pathovars tested.