CLONING AND EXPRESSION OF VARIOUS STAPHYLOCOCCAL GENES ENCODING UREASE IN STAPHYLOCOCCUS-CARNOSUS

被引：6

作者：

JOSE, J

CHRISTIANS, S

ROSENSTEIN, R

GOTZ, F

KALTWASSER, H

机构：

[1] UNIV TUBINGEN,W-7400 TUBINGEN 1,GERMANY

[2] ZENTRUM MOLEK BIOL,HEIDELBERG,GERMANY

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 80卷 / 2-3期

关键词：

UREASE; STAPHYLOCOCCUS; MOLECULAR CLONING; NICKEL;

D O I：

10.1016/0378-1097(91)90609-E

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The urease genes from Staphylococcus xylosus C2a, Staphylococcus aureus U500, and S. aureus Newman were cloned in Staphylococcus carnosus using the plasmid vectors pCA43 and pCA44. The resulting respective recombinant plasmids pUra 402, pUraUH66, and pUra17 contained chromosomal DNA fragments with sizes of 5.6, 5.8, and 6.8 kb, respectively. Investigations on urease expression of the donor and recombinant strains in media with various nitrogen sources revealed that S. xylosus C2a produced urease constitutively at the highest specific activity. All of the recombinant strains had significantly lower urease activities than their DNA-donor strains. The nickel-dependence of urease was demonstrated in S. aureus U500 by a plate diffusion assay.

引用

页码：277 / 282

页数：6

共 25 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1515

[2] JACK-BEAN-UREASE - THE 1ST NICKEL ENZYME [J].