STAUROSPORINE INDUCES A NEURONAL PHENOTYPE IN SH-SY5Y HUMAN NEUROBLASTOMA-CELLS THAT RESEMBLES THAT INDUCED BY THE PHORBOL ESTER 12-O-TETRADECANOYL PHORBOL-13 ACETATE (TPA)

被引：58

作者：

JALAVA, A ^{[1
]}

HEIKKILA, J ^{[1
]}

LINTUNEN, M ^{[1
]}

AKERMAN, K ^{[1
]}

PAHLMAN, S ^{[1
]}

机构：

[1] UNIV HOSP UPPSALA,DEPT PATHOL,S-75185 UPPSALA,SWEDEN

来源：

FEBS LETTERS | 1992年 / 300卷 / 02期

关键词：

STAUROSPORINE; PROTEIN KINASE C; NEUROBLASTOMA DIFFERENTIATION; NEUROPEPTIDE-Y; TYROSINE HYDROXYLASE; GAP-43; MYC;

D O I：

10.1016/0014-5793(92)80176-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Treatment of SH-SY5Y human neuroblastoma cells with the protein kinase inhibitor staurosporine, induced both morphological and functional differentiation in these cells. The effects of staurosporine were comparable to those induced by the protein kinase C (PKC) activator, 12-O tetradecanoyl phorbol 13-acetate (TPA), with respect to induction of neuronal differentiation, i.e. neurite outgrowth, inhibition of DNA synthesis, induction and down-regulation of c-myc protein expression, induction of mRNA for both neuropeptide Y (NPY) and growth associated protein 43 (GAP-43) and stimulation of tyrosine hydroxylase expression. Staurosporine failed to translocate PKC to the membrane fraction or to stimulate phosphorylation of the endogenous PKC substrate M(r) 80,000 (p80). Instead, staurosporine inhibited TPA-induced phosphorylation of p80.

引用

页码：114 / 118

页数：5

共 33 条

[1] NEUROPEPTIDE-Y DISTRIBUTION IN HUMAN-BRAIN [J].