A HUMAN HOMOLOG OF THE YEAST REPLICATION PROTEIN CDC21 - INTERACTIONS WITH OTHER MCM-PROTEINS

被引：84

作者：

MUSAHL, C ^{[1
]}

SCHULTE, D ^{[1
]}

BURKHART, R ^{[1
]}

KNIPPERS, R ^{[1
]}

机构：

[1] UNIV KONSTANZ,DIV BIOL,D-78434 CONSTANCE,GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 230卷 / 03期

关键词：

NUCLEAR PROTEINS; MCM PROTEINS; CDC21; PROTEIN; MCM2;

D O I：

10.1111/j.1432-1033.1995.tb20660.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present the amino acid sequence of the human homologue of the yeast replication protein Cdc21, a member of the Mcm family of nuclear proteins. Specific antibodies, raised against protein hCdc21, were used to investigate the expression of the protein through the cell cycle. The protein is highly phosphorylated in mitotic cells. The phosphorylated form of protein hCd21 appears to be less tightly bound to nuclear structures than the underphosphorylated form suggesting that phosphorylation/dephosphorylation reactions may determine the nuclear distribution of the protein. Protein hCdc21 forms a stable trimeric complex with two novel human Mcm proteins, p85Mcm and p105Mcm. Protein BM28/Mcm2 is more loosely associated with the trimeric hCdc21 complex.

引用

页码：1096 / 1101

页数：6

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