DESENSITIZATION OF DELAYED-TYPE HYPERSENSITIVITY IN MICE - POSSIBLE INVOLVEMENT OF INTERLEUKIN 2-DEPENDENT REGULATORY MECHANISMS IN DESENSITIZED MICE

The systemic injection of high doses of antigen into a previously immunized animal results in a state of transient anergy with respect to cell-mediated immune responses. This phenomenon is known as desensitization. The results presented here demonstrated that serum interleukin 2 (IL-2) activity was found transiently in desensitized mice at early stage (3 hr after the challenge). Subsequently, these mice could not develop in vivo (footpad swelling) and in vitro (lymphocyte proliferation) manifestations of cell-mediated immune responses 1 day after the challenge. Antigen-nonspecific and specific suppression of IL-2 production was observed in desensitized mice. The serum from 3 hr-desensitized mice containing endogenous IL-2 activity showed a marked suppressive effect on IL-2 production. Exposure of lymph node cells to IL-2 was capable of inhibiting IL-2 production in vitro. Additionally, in vivo administration of exogenous IL-2 into preimmunized mice led to the failure of development of footpad responses to antigen. These results suggest that IL-2-dependent regulatory mechanisms of T cell-mediated immune responses play an important role in the immunosuppression of desensitized mice. © 1990.