RAPID-DETERMINATION OF MICROORGANISMS USING A FLOW-INJECTION SYSTEM

被引：17

作者：

DING, T

SCHMID, RD

机构：

[1] GBF - Gesellschaft für Biotechnologische Forschung, Department of Enzyme Technology, D-3300 Braunschweig

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 234卷 / 01期

关键词：

D O I：

10.1016/S0003-2670(00)83564-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An automated method for the rapid determination of microorganisms using a flow-injection system is presented. Electrochemical measurement of a mediator reduced by microbial metabolism allowed the determination of fungi and bacteria in a few minutes. The lowest detection limit was 5 × 106 colony-forming units (cfu) ml-1 for Escherichia coli. Correlation between the flow-injection method and standard microbiological methods was excellent (r = 0.997, n = 4 for Beauveria bassiana; r = 0.997, n = 7 for E. coli). The flow-injection system was applied to the on-line control of an E. coli cultivation. © 1990.

引用

页码：247 / 251

页数：5

共 25 条

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