SENSITIVE ENZYME-IMMUNOASSAY FOR HUMAN CU/ZN SUPEROXIDE-DISMUTASE

被引：50

作者：

KUROBE, N ^{[1
]}

SUZUKI, F ^{[1
]}

OKAJIMA, K ^{[1
]}

KATO, K ^{[1
]}

机构：

[1] INST DEV RES,DEPT BIOCHEM,AICHI PREFECTURAL COLONY,KASUGAI,AICHI 48003,JAPAN

来源：

CLINICA CHIMICA ACTA | 1990年 / 187卷 / 01期

关键词：

Down syndrome; Immunoassay; Superoxide dismutase;

D O I：

10.1016/0009-8981(90)90257-S

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A highly sensitive enzyme immunoassay method for measurement of human Cu/Zn superoxide dismutase (SOD) was established. Antisera were raised in rabbits by injecting SOD purified from human erythrocytes, and antibodies to SOD were purified by the use of Cu/Zn SOD-coupled Sepharose 4B. The assay system consisted of polystyrene balls with immobilized monospecific antibody F(ab')2 fragments and the same antibody Fab' fragments labeled with β-d-galactosidase from Escherichia coli. The assay was highly sensitive, and the minimum detection limit was 3 pg or 0.1 fmol/assay tube. Serum Cu/Zn SOD levels of normal healthy subjects (36.3 ± 15.6, n = 120, 16-64 years old) were not related to age and sex. It was confirmed that Cu/Zn SOD levels in erythrocytes and blood plasma were significantly enhanced in patients with Down syndrome. Immunoreactive Cu/Zn SOD was detectable in all the tissues examined and was present at high concentrations in brain, adrenal gland, liver, heart and kidney. © 1990.

引用

页码：11 / 20

页数：10

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