THE NUCLEOTIDE-SEQUENCE OF THE LUXA AND LUXB GENES OF XENORHABDUS-LUMINESCENS HM AND A COMPARISON OF THE AMINO-ACID-SEQUENCES OF LUCIFERASES FROM 4 SPECIES OF BIOLUMINESCENT BACTERIA

被引：31

作者：

JOHNSTON, TC ^{[1
]}

RUCKER, EB ^{[1
]}

COCHRUM, L ^{[1
]}

HRUSKA, KS ^{[1
]}

VANDEGRIFT, V ^{[1
]}

机构：

[1] MURRAY STATE UNIV,DEPT CHEM,MURRAY,KY 42071

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92106-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The luxA and luxB genes of bioluminescent bacteria encode the α and β subunits of luciferase, respectively. Sequences of the luxA and luxB genes of Xenorhabdus luminescens, the only terrestrial bioluminescent bacterium known, were determined and the amino acid sequence of luciferase deduced. The α subunit was found to contain 360 amino acids and has a calculated molecular weight of 41,005 Da, while the β subunit contains 327 amino acids and has a calculated molecular weight of 37,684 Da. Alignment of this luciferase with the luciferases of three marine bacteria showed 196 (or 55%) conserved residues in the α subunit and 114 (or 35%) conserved residues in the β subunit. The highest degree of homology between any two species was between the luciferases of X. luminescens and Vibrio harveyi with 84% identity in the α subunits and 59% identity in the β subunits. © 1990.

引用

页码：407 / 415

页数：9

共 31 条

[1] SITE-DIRECTED MUTAGENESIS OF BACTERIAL LUCIFERASE - ANALYSIS OF THE ESSENTIAL THIOL [J].