CHARACTERIZATION OF THE VPG DSRNA LINKAGE OF INFECTIOUS PANCREATIC NECROSIS VIRUS

被引：59

作者：

CALVERT, JG ^{[1
]}

NAGY, E ^{[1
]}

SOLER, M ^{[1
]}

DOBOS, P ^{[1
]}

机构：

[1] UNIV GUELPH,COLL BIOL SCI,DEPT MICROBIOL,GUELPH N1G 2W1,ONTARIO,CANADA

来源：

JOURNAL OF GENERAL VIROLOGY | 1991年 / 72卷

关键词：

D O I：

10.1099/0022-1317-72-10-2563

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

By the use of strong denaturing agents, a genome-linked protein (VPg)-RNA complex was purified from infectious pancreatic necrosis virus. Ribonuclease treatment of I-125-labelled VPg-RNA released a 90K polypeptide identical to the minor structural polypeptide VPI (the putative RNA polymerase), as determined by peptide mapping. The polypeptide is linked to the RNA by a serine-5' GMP phosphodiester bond. The results identify birnaviruses as the only dsRNA viruses with a VPg, the size of which is the largest of the VPgs of RNA viruses.

引用

页码：2563 / 2567

页数：5

共 20 条

[1] DROSOPHILA-X VIRUS-RNA POLYMERASE - TENTATIVE MODEL FOR INVITRO REPLICATION OF THE DOUBLE-STRANDED VIRION RNA
BERNARD, J
[J]. JOURNAL OF VIROLOGY, 1980, 33 (02) : 717 - 723
[2] BROWN F, 1986, INTERVIROLOGY, V25, P140
[3] Daubert S. D., 1984, METHOD VIROL, P347
[4] PEPTIDE MAP COMPARISON OF INFECTIOUS PANCREATIC NECROSIS VIRUS-SPECIFIC POLYPEPTIDES
DOBOS, P
ROWE, D
[J]. JOURNAL OF VIROLOGY, 1977, 24 (03) : 805 - 820
[5] THE NUCLEOTIDE-SEQUENCE OF INFECTIOUS PANCREATIC NECROSIS VIRUS (IPNV) DSRNA SEGMENT-A REVEALS ONE LARGE ORF ENCODING A PRECURSOR POLYPROTEIN
DUNCAN, R
DOBOS, P
[J]. NUCLEIC ACIDS RESEARCH, 1986, 14 (14) : 5934 - 5934
[6] SYNTHESIS OF THE INFECTIOUS PANCREATIC NECROSIS VIRUS POLYPROTEIN, DETECTION OF A VIRUS-ENCODED PROTEASE, AND FINE-STRUCTURE MAPPING OF GENOME SEGMENT-A CODING REGIONS
DUNCAN, R
NAGY, E
KRELL, PJ
DOBOS, P
[J]. JOURNAL OF VIROLOGY, 1987, 61 (12) : 3655 - 3664
[7] SEQUENCE-ANALYSIS OF INFECTIOUS PANCREATIC NECROSIS VIRUS GENOME SEGMENT-B AND ITS ENCODED VP1 PROTEIN - A PUTATIVE RNA-DEPENDENT RNA-POLYMERASE LACKING THE GLY ASP ASP MOTIF
DUNCAN, R
MASON, CL
NAGY, E
LEONG, JA
DOBOS, P
[J]. VIROLOGY, 1991, 181 (02) : 541 - 552
[8] AN ESSENTIAL ARGININE RESIDUE FOR INITIATION OF PROTEIN-PRIMED DNA-REPLICATION
HSIEH, JC
YOO, SK
ITO, JS
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) : 8665 - 8669
[9] BIRNAVIRUS PRECURSOR POLYPROTEIN IS PROCESSED IN ESCHERICHIA-COLI BY ITS OWN VIRUS-ENCODED POLYPEPTIDE
JAGADISH, MN
STATON, VJ
HUDSON, PJ
AZAD, AA
[J]. JOURNAL OF VIROLOGY, 1988, 62 (03) : 1084 - 1087
[10] TWO-DIMENSIONAL SEPARATION OF PHOSPHOAMINO ACIDS FROM NUCLEOSIDE MONOPHOSPHATES
MANAI, M
COZZONE, AJ
[J]. ANALYTICAL BIOCHEMISTRY, 1982, 124 (01) : 12 - 18

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