A POTENTIOMETRIC STUDY INTO THE STABILITY OF THE SCHIFF-BASE FORMED BETWEEN PYRIDOXAL 5'-PHOSPHATE AND HEXYLAMINE IN WATER DIOXANE MIXTURES

被引：5

作者：

DELPINO, IMP ^{[1
]}

SANCHEZRUIZ, JM ^{[1
]}

机构：

[1] UNIV GRANADA,FAC CIENCIAS,DEPT QUIM FIS,E-18071 GRANADA,SPAIN

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2 | 1993年 / 03期

关键词：

D O I：

10.1039/p29930000573

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We have developed a fast and accurate potentiometric method to characterize the pH-dependent, Schiff's base formation equilibria in water-organic cosolvent mixtures. This method has been applied to the reaction between pyridoxal 5'-phosphate and hexylamine in water-dioxane. We have found that, at neutral and basic pH, the Schiff's base is more stable in the lower polarity media. It is suggested that this is a secondary effect, due to the fact that Schiff 's base formation is accompanied by charge neutralization.

引用

页码：573 / 579

页数：7

共 24 条

[1]

[Anonymous], 1969, DATA REDUCTION ERROR

[2]

CORTIJO M, 1988, J BIOL CHEM, V263, P17960

[3] CRITERIA TO RECOGNIZE STRUCTURE AND MICROPOLARITY OF PYRIDOXAL 5'-PHOSPHATE-BINDING SITES IN PROTEINS [J].

CORTIJO, M ;

JIMENEZ, JS ;

LLOR, J .

BIOCHEMICAL JOURNAL, 1978, 171 (02) :497-500

[4]

DELPINO IMP, 1991, J CHEM EDUC, V68, P944

[5] INFLUENCE OF POLARITY OF THE MEDIUM ON THE CATALYSIS OF FORMATION AND STABILITY OF THE SCHIFF-BASE FROM PYRIDOXAL 5'-PHOSPHATE AND NORMAL-HEXYLAMINE [J].

DELVADO, MAG ;

ECHEVARRIA, GR ;

GARCIAESPANTALEON, A ;

DONOSO, J ;

MUNOZ, F ;

BLANCO, FG .

JOURNAL OF MOLECULAR CATALYSIS, 1988, 44 (03) :313-322

[6]

HARNED HS, 1958, PHYSICAL CHEM ELECTR

[7] LIGAND-BINDING TO MACROMOLECULES - ALLOSTERIC AND SEQUENTIAL MODELS OF COOPERATIVITY [J].

HESS, VL ;

SZABO, A .

JOURNAL OF CHEMICAL EDUCATION, 1979, 56 (05) :289-293

[8]

KALLEN RG, 1986, TRANSAMINASES, P37

[9] MODEL PROCESS FOR MEASUREMENT OF SOLVENT POLARITY [J].

LLOR, J ;

CORTIJO, M .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1977, (09) :1111-1113

[10] POLAROGRAPHIC-REDUCTION OF A PYRIDOXAL 5'-PHOSPHATE SCHIFFS BASE [J].

LLOR, J ;

CORTIJO, M .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1978, (05) :409-411

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