SEROLOGICAL SURVEY OF CANINE-DISTEMPER VIRUS-INFECTION USING ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：22

作者：

GEMMA, T ^{[1
]}

MIYASHITA, N ^{[1
]}

SHIN, YS ^{[1
]}

OKITA, M ^{[1
]}

MORI, T ^{[1
]}

IWATSUKI, K ^{[1
]}

MIKAMI, T ^{[1
]}

KAI, C ^{[1
]}

机构：

[1] UNIV TOKYO,FAC AGR,DEPT VET MICROBIOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1995年 / 57卷 / 04期

关键词：

CANINE DISTEMPER VIRUS; ELISA; SERUM NEUTRALIZATION TEST;

D O I：

10.1292/jvms.57.761

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

For detection of antibodies against canine distemper virus (CDV), an enzyme-linked immunosorbent assay (ELISA) was developed using a crude extract from cells infected with the Onderstepoort strain of CDV as antigen. Twenty-six sera from dogs experimentally vaccinated with the Snyder-Hill strain of CDV were compared by ELISA and a standard virus neutralization (NV) test. Since a good correlation between the titers obtained by both tests was observed, ELISA was considered to be a rapid and reliable method for a serological survey of CDV infection. When a total of 167 sera from dogs suspected of canine distemper under natural conditions were examined by the ELISA, 29 of the sera (17%) were found to have low VN antibody titers and high ELISA titers. The reason for the discrepancy in the titers was discussed.

引用

页码：761 / 763

页数：3

共 12 条

[1]

APPEL MJG, 1969, AM J VET RES, V30, P1167

[2]

APPEL MJG, 1987, VIRUS INFECTIONS VER, V1, P133

[3] BACULOVIRUS EXPRESSION OF THE NUCLEOPROTEIN GENE OF MEASLES-VIRUS AND UTILITY OF THE RECOMBINANT PROTEIN IN DIAGNOSTIC ENZYME IMMUNOASSAYS [J].

HUMMEL, KB ;

ERDMAN, DD ;

HEATH, J ;

BELLINI, WJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (11) :2874-2880

[4] USE OF B95A CELLS FOR ISOLATION OF CANINE-DISTEMPER VIRUS FROM CLINICAL CASES [J].

KAI, C ;

OCHIKUBO, F ;

OKITA, M ;

IINUMA, T ;

MIKAMI, T ;

KOBUNE, F ;

YAMANOUCHI, K .

JOURNAL OF VETERINARY MEDICAL SCIENCE, 1993, 55 (06) :1067-1070

[5]

LAUDER I. M., 1954, Veterinary Record, V66, P623

[6]

LAUDER I. M., 1954, Veterinary Record, V66, P607

[7] A COMPETITIVE ELISA USING ANTI-N MONOCLONAL-ANTIBODIES FOR SPECIFIC DETECTION OF RINDERPEST ANTIBODIES IN CATTLE AND SMALL RUMINANTS [J].

LIBEAU, G ;

DIALLO, A ;

CALVEZ, D ;

LEFEVRE, PC .

VETERINARY MICROBIOLOGY, 1992, 31 (2-3) :147-160

[8] ANTIBODY-RESPONSES TO VIRION POLYPEPTIDES IN GNOTOBIOTIC DOGS INFECTED WITH CANINE-DISTEMPER VIRUS [J].

MIELE, JA ;

KRAKOWKA, S .

INFECTION AND IMMUNITY, 1983, 41 (02) :869-871

[9]

MOLLER MB, 1991, J VET DIAGN INVEST, V3, P3

[10]

NOON KF, 1980, AM J VET RES, V41, P605

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