INVOLVEMENT OF RHO IN GTP-GAMMA-S-INDUCED ENHANCEMENT OF PHOSPHORYLATION OF 20 KDA MYOSIN LIGHT-CHAIN IN VASCULAR SMOOTH-MUSCLE CELLS - INHIBITION OF PHOSPHATASE-ACTIVITY

被引：161

作者：

NODA, M

YASUDAFUKAZAWA, C

MORIISHI, K

KATO, T

OKUDA, T

KUROKAWA, K

TAKUWA, Y

机构：

[1] UNIV TOKYO,FAC MED,DEPT CARDIOVASC BIOL,BUNKYO KU,TOKYO 113,JAPAN

[2] UNIV TOKYO,FAC MED,DEPT INTERNAL MED,BUNKYO KU,TOKYO 113,JAPAN

[3] NATL INST HLTH,TOKYO 162,JAPAN

来源：

FEBS LETTERS | 1995年 / 367卷 / 03期

关键词：

RHO; MYOSIN LIGHT CHAIN PHOSPHATASE GTP-GAMMA-S; SMOOTH MUSCLE;

D O I：

10.1016/0014-5793(95)00573-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In beta-escin-permeabilized cultured pig aortic smooth muscle cells GTP gamma S dose-dependently enhances Ca2+-induced, wortmannin-sensitive phosphorylation of 20 kDa myosin light chain (MLC(20)), GTP gamma S does not potentiate thiophosphorylation of MLC(20), but does inhibit its dephosphorylation, Pretreatment with C, botulinum exotoxin C-3, which specifically ADP-ribosylates and inactivates the rho family of the small molecular weight G proteins, completely abolishes the effects of GTP gamma S, These results indicate that rite is involved in the GTP gamma S-induced enhancement of Ca2+-dependent MLC(20) phosphorylation in aortic smooth muscle cells, and strongly suggest that this effect of rho is due to inhibition of protein phosphatase activity toward MLC(20).

引用

页码：246 / 250

页数：5

共 25 条

[1] THE CONTROL OF PROTEIN PHOSPHATASE-1 BY TARGETING SUBUNITS - THE MAJOR MYOSIN PHOSPHATASE IN AVIAN SMOOTH-MUSCLE IS A NOVEL FORM OF PROTEIN PHOSPHATASE-1 [J].