DETERMINATION OF QUINOLINIC ACID IN HUMAN URINE BY LIQUID-CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION

被引：50

作者：

MAWATARI, K

OSHIDA, K

IINUMA, F

WATANABE, M

机构：

[1] Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 302卷 / 2-3期

关键词：

FLUOROMETRY; LIQUID CHROMATOGRAPHY; QUINOLINIC ACID; POSTCOLUMN UV IRRADIATION; HYDROGEN PEROXIDE;

D O I：

10.1016/0003-2670(94)00493-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A system has been developed for the: determination of quinolinic acid in human urine using liquid chromatography with fluorimetric detection. The compound is separated by reversed-phase chromatography using a 35 mM potassium dihydrogenphosphate solution (adjusted to pH 3.8 with 0.2 M citric acid) containing 350 mM hydrogen peroxide and 0.05 mM tetramethylammonium hydroxide as a mobile phase. The compound in the column effluent is irradiated with ultraviolet light to produce fluorescence. This fluorescence is monitored with excitation at 326 nm and emission at 380 nm. The calibration graph for quinolinic acid is linear over the range 0.36-68.8 nmol ml(-1) upon injecting 10 mu l of the standard solution. Pretreatment of urine was achieved by dilution and filtration only. The mean recovery of quinolinic acid from urine is more than 95%.

引用

页码：179 / 183

页数：5

共 18 条

[1] NEUROTOXICITY OF TRYPTOPHAN-METABOLITES [J].

BOEGMAN, RJ ;

JHAMANDAS, K ;

BENINGER, RJ .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1990, 585 :261-273

[2] VITAMIN B6 DEPLETION IN MAN - URINARY EXCRETION OF QUINOLINIC ACID AND NIACIN METABOLITES [J].

BROWN, RR ;

YESS, N ;

PRICE, JM ;

LINKSWILER, H ;

SWAN, P ;

HANKES, LV .

JOURNAL OF NUTRITION, 1965, 87 (04) :419-+

[3]

CHAMBERLIN BA, 1987, CLIN CHEM, V33, P572

[4] NEUROACTIVE METABOLITES OF L-TRYPTOPHAN, SEROTONIN AND QUINOLINIC ACID, IN STRIATAL EXTRACELLULAR FLUID - EFFECT OF TRYPTOPHAN LOADING [J].

DURING, MJ ;

FREESE, A ;

HEYES, MP ;

SWARTZ, KJ ;

MARKEY, SP ;

ROTH, RH ;

MARTIN, JB .

FEBS LETTERS, 1989, 247 (02) :438-444

[5] A RADIOENZYMATIC ASSAY FOR QUINOLINIC ACID [J].

FOSTER, AC ;

OKUNO, E ;

BROUGHER, DS ;

SCHWARCZ, R .

ANALYTICAL BIOCHEMISTRY, 1986, 158 (01) :98-103

[6] QUANTIFICATION OF QUINOLINIC ACID IN RAT-BRAIN, WHOLE-BLOOD, AND PLASMA BY GAS-CHROMATOGRAPHY AND NEGATIVE CHEMICAL IONIZATION MASS-SPECTROMETRY - EFFECTS OF SYSTEMIC L-TRYPTOPHAN ADMINISTRATION ON BRAIN AND BLOOD QUINOLINIC ACID CONCENTRATIONS [J].

HEYES, MP ;

MARKEY, SP .

ANALYTICAL BIOCHEMISTRY, 1988, 174 (01) :349-359

[7] FLUOROMETRIC DETERMINATION OF KYNURENIC ACID IN HUMAN-SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COUPLED WITH POSTCOLUMN PHOTOCHEMICAL-REACTION WITH HYDROGEN-PEROXIDE [J].

MAWATARI, K ;

IINUMA, F ;

WATANABE, M .

ANALYTICAL SCIENCES, 1988, 4 (02) :195-197

[8] FLUOROMETRIC-DETERMINATION OF URINARY KYNURENIC ACID BY FLOW-INJECTION ANALYSIS EQUIPPED WITH A BYPASS LINE [J].

MAWATARI, K ;

IINUMA, F ;

WATANABE, M .

ANALYTICAL BIOCHEMISTRY, 1990, 190 (01) :88-91

[9] DETERMINATION OF NICOTINIC-ACID AND NICOTINAMIDE IN HUMAN SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH POSTCOLUMN ULTRAVIOLET-IRRADIATION AND FLUORESCENCE DETECTION [J].

MAWATARI, K ;

IINUMA, F ;

WATANABE, M .

ANALYTICAL SCIENCES, 1991, 7 (05) :733-736

[10] DETERMINATION OF URINARY QUINOLINIC ACID BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

PATTERSON, JI ;

BROWN, RR .

JOURNAL OF CHROMATOGRAPHY, 1980, 182 (3-4) :425-429

← 1 2 →