A NOVEL FUNCTION OF HAPTOGLOBIN - HAPTOGLOBIN-HEMOGLOBIN COMPLEX INDUCES APOPTOSIS OF HEPATOCARCINOMATOUS HEP 3B CELLS

被引：19

作者：

KIM, IK ^{[1
]}

LEE, JH ^{[1
]}

KIM, HS ^{[1
]}

KWON, OJ ^{[1
]}

SHIM, BS ^{[1
]}

机构：

[1] CATHOLIC UNIV MED COLL,DEPT BIOCHEM,SOCHO KU,SEOUL 137701,SOUTH KOREA

来源：

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 1995年 / 55卷 / 06期

关键词：

DNA FRAGMENTATION; HEP G2 CELLS; HEPATOMA; TRANSGLUTAMINASE;

D O I：

10.3109/00365519509075391

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Haptoglobin-haemoglobin complex (Cx) showed a cytotoxic effect on the growth of Hep 3B (human hepatocellular carcinoma) cells, dose dependently. The antiproliferative effect of Cx on the multiplication of Hep 3B cells was augmented by the presence of prostaglandin (PC) D-2. Antihuman Hb IgG abolished the effect of Cx, dose-dependently, which indicates that the antiproliferative effect of Cx really is exerted by Cx. Hep 3B cells treated with Cx showed the characteristic biochemical changes of apoptosis, such as DNA fragmentation which was blocked by pretreatment with cycloheximide, and the increase of transglutaminase expression. Thus, the antiproliferative effect of Cx against Hep 3B cells occurs via the typical apoptotic pathway.

引用

页码：529 / 535

页数：7

共 43 条

[1] BADR KF, 1994, HARRISONS PRINCIPLES, P1320
[2] Boyd W., 1966, SPONTANEOUS REGRESSI
[3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4] BRANDSLUND I, 1980, HAEMOSTASIS, V9, P193
[5] ENDOTOXIN-INDUCED SERUM FACTOR THAT CAUSES NECROSIS OF TUMORS
CARSWELL, EA
OLD, LJ
KASSEL, RL
GREEN, S
FIORE, N
WILLIAMSON, B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (09) : 3666 - 3670
[6] STUDIES ON REACTION OF HAPTOGLOBIN WITH HAEMOGLOBIN AND HAEMOGLOBIN CHAINS .I. STOICHIOMETRY AND AFFINITY
CHIANCONE, E
ALFSEN, A
IOPPOLO, C
VECCHINI, P
AGRO, AF
WYMAN, J
ANTONINI, E
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1968, 34 (02) : 347 - +
[7] HAPTOGLOBIN - A NATURAL BACTERIOSTAT
EATON, JW
BRANDT, P
MAHONEY, JR
LEE, JT
[J]. SCIENCE, 1982, 215 (4533) : 691 - 693
[8] STUDIES ON THE STRUCTURE OF HEMOGLOBIN .1. PHYSICOCHEMICAL PROPERTIES OF HUMAN GLOBIN
FANELLI, AR
ANTONINI, E
CAPUTO, A
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1958, 30 (03) : 608 - 615
[9] APOPTOTIC HEPATOCYTES BECOME INSOLUBLE IN DETERGENTS AND CHAOTROPIC AGENTS AS A RESULT OF TRANSGLUTAMINASE ACTION
FESUS, L
THOMAZY, V
AUTUORI, F
CERU, MP
TARCSA, E
PIACENTINI, M
[J]. FEBS LETTERS, 1989, 245 (1-2) : 150 - 154
[10] PURIFICATION OF DNA FROM FORMALDEHYDE FIXED AND PARAFFIN EMBEDDED HUMAN-TISSUE
GOELZ, SE
HAMILTON, SR
VOGELSTEIN, B
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 130 (01) : 118 - 126

← 1 2 3 4 5 →