Neurone decapping characterization by atomic force microscopy: A topological systematic analysis

被引：8

作者：

De Stasio, G

Cricenti, A

Generosi, R

Mercanti, D

Ciotti, MT

Casalbore, P

Margaritondo, G

Perfetti, P

机构：

[1] CNR, IST STRUTTURA MAT, I-00044 FRASCATI, ROMA, ITALY

[2] CNR, IST NEUROBIOL, I-00100 ROME, ITALY

[3] CNR, IST BIOL CELLULARE, I-00100 ROME, ITALY

来源：

NEUROREPORT | 1995年 / 7卷 / 01期

关键词：

rat cerebellar granule cells; neurones; decapping; atomic force microscopy (AFM); surface sensitive techniques;

D O I：

10.1097/00001756-199512290-00015

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

WE tested a new approach to cell decapping on rat cerebellar neurones, and observed its effects on cell topography by atomic force microscopy (AFM). The results clearly demonstrate the effectiveness of our decapping approach, and also the ability of AFM to reveal fine details of the decapped cells. Specifically, varying the conditions and duration of the decapping process modifies the extent of the decapping. Such a method can be used to investigate the cytoplasm with surface sensitive techniques.

引用

页码：65 / 68

页数：4

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