CHROMOSOMAL MAPPING OF T-DNA INSERTS IN TRANSGENIC PETUNIA BY IN-SITU HYBRIDIZATION

被引：45

作者：

WANG, J

LEWIS, ME

WHALLON, JH

SINK, KC

机构：

[1] MICHIGAN STATE UNIV, DEPT HORT, E LANSING, MI 48824 USA

[2] MICHIGAN STATE UNIV, DEPT CROP & SOIL SCI, E LANSING, MI 48824 USA

来源：

TRANSGENIC RESEARCH | 1995年 / 4卷 / 04期

关键词：

PETUNIA; T-DNA; IN SITU HYBRIDIZATION; TAGGING;

D O I：

10.1007/BF01969117

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The chromosomal location of T-DNA inserts in ten independently derived and confirmed transgenic plants of P. hybrida was detected by in situ hybridization. Nine transgenic plants had the T-DNA inserts at single sites distributed among each of the seven chromosomes; in one plant the T-DNA inserts were detected on two different chromosomes. Although the T-DNA inserts were integrated randomly among the chromosomes, seven of the 11 total inserts were located at or near the telomere. Thus, T-DNA inserts appear to have potential for tagging chromosomes and chromosome fragments.

引用

页码：241 / 246

页数：6

共 22 条

[1]

Ambros P.F., Matzke A.J.M., Matzke M.A., Localization of Agrobacterium rhizogenes T-DNA in plant chromosome by in situ hybridization, EMBO J, 5, pp. 2073-7, (1986)

[2]

Andre D., Colau D., Schell J., Van Montagu M., Hernalsteens J.P., Gene tagging in plants by a T-DNA insertion mutagen that generates APH(3′)II-plant gene fusions, Molecular and General Genetics MGG, 204, pp. 512-8, (1986)

[3]

Becker D., Kemper E., Schell J., Masterson R., New plant binary vectors with selectable markers located proximal to the left T-DNA border, Plant Mol. Biol., 20, pp. 1195-7, (1992)

[4]

Dellaporta S.L., Wood J., Hicks J.B., Maize DNA miniprep, Molecular Biology of Plants, a Laboratory Manual, pp. 36-37, (1985)

[5]

Fraley R.T., Rogers S.G., Horsch R.B., Eicholtz D.A., Flick J.S., Fink C.L., Hoffman N.L., Sanders P.R., The SEV system: a new disarmed Ti plasmid vector for plant transformation, Bio/Technology, 3, pp. 629-35, (1985)

[6]

Gustafson J.P., Butler E., McIntyre C.L., Physical mapping of a low-copy DNA sequence in rye (Secale cereale L.), Proc. Natl Acad. Sci. USA, 87, pp. 1899-1902, (1990)

[7]

Horsch R.B., Fry J.E., Hoffman N.L., Eichholtz D.A., Rogers S.G., Fraley R.T., Transferring genes into plants, Science, 227, pp. 1229-31, (1985)

[8]

Jhanwar S.C., Neel B.G., Hayward W.S., Chaganti R.S.K., Localization of c-ras oncogene family on human germ line chromosomes, Proc. Natl Acad. Sci. USA, 80, pp. 4794-7, (1983)

[9]

Johnson C., Singer R.H., Lawrence J.B., Fluorescent detection of nuclear RNA and DNA: implications for genome organization, Methods Cell Biology, 35, pp. 73-99, (1991)

[10]

Klee H.J., Horsch R.B., Rogers S.G., Agrobacterium mediated plant transformation and its further applications to plant biology, Annual Review of Plant Physiology, 38, pp. 467-86, (1987)

← 1 2 3 →