LACRIMAL PROTEIN SECRETION - COMPARISON OF YOUNG AND OLD RATS

被引:42
作者
BROMBERG, BB
WELCH, MH
机构
[1] UNIV NEW ORLEANS, DEPT BIOL SCI, NEW ORLEANS, LA 70148 USA
[2] LOUISIANA STATE UNIV, MED CTR, CTR MED, CTR EYE, NEW ORLEANS, LA 70112 USA
关键词
D O I
10.1016/0014-4835(85)90015-6
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
The in vitro protein secretory response of lacrimal glands from healthy 4- and 24-mo.-old F344 rats was measured. Tissue fragments were incubated in a 0.2 ml perifusion chamber and stimulated to secrete protein by a 4-min bolus of 0.01 mM carbachol. Fractions of the perifusate were collected and assayed for protein concentration and peroxidase activity. The response to carbachol was a well-defined peak of secreted protein that included peroxidase. The response was inhibited by 1.0 .mu.M atropine. No differences were found between the responses of male and female glands. The peak of protein secretion by the young glands contained 263.9 .+-. 71.8 .mu.g g tissue-1 compared to 175.4 .+-. 56.5 .mu.g g tissues-1 for the old glands (P < 0.005). While the mean activity of the secreted peroxidase was similar in the 2 age groups, the aged glands were significantly more variable in their response. The F344 rat may provide a useful model for studying the normal age-dependent changes that occur in lacrimal gland.
引用
收藏
页码:313 / 320
页数:8
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