EXPRESSION OF RECOMBINANT HUMAN PLASMINOGEN AND AGLYCOPLASMINOGEN IN HELA-CELLS

被引：5

作者：

BROWNE, MJ

CHAPMAN, CG

DODD, I

CAREY, JE

LAWRENCE, GMP

MITCHELL, DL

ROBINSON, JH

机构：

[1] Department of Biotechnology, SmithKline Beecham, Yew Tree Bottom Road, Epsom

来源：

FIBRINOLYSIS | 1991年 / 5卷 / 04期

关键词：

PLASMINOGEN; GLYCOSYLATION; STREPTOKINASE; THROMBOLYTIC THERAPY;

D O I：

10.1016/0268-9499(91)90010-2

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

cDNA clones encoding human plasminogen (PLGN) and aglyco (Asn289 --> Gln, Thr346 --> Ala) plasminogen (APLGN) were expressed under the control of the Rous sarcomavirus long terminal repeat (RSVLTR) in a HeLa cell transient expression system. Harvest medium was analysed for the presence of recombinant PLGN using three streptokinase (SK)-dependent methods; (1) a direct S2251 amidolytic assay; (2) a plasminogen activator assay and (3) fibrin zymography after electrophoresis. Our results are consistent with the expression of authentic PLGN (both type I and type II) and APLGN.

引用

页码：257 / 260

页数：4

共 23 条

[1] THE FIBRIN PLATE METHOD FOR ESTIMATING FIBRINOLYTIC ACTIVITY [J].