EFFECTS OF CALCIUM-IONS ON PROTEOLYTIC PROCESSING OF HIV-1 GP160 PRECURSOR AND ON CELL-FUSION

被引：23

作者：

MOULARD, M ^{[1
]}

MONTAGNIER, L ^{[1
]}

BAHRAOUI, E ^{[1
]}

机构：

[1] INST PASTEUR,CNRS,URA 1157,F-75724 PARIS 15,FRANCE

来源：

FEBS LETTERS | 1994年 / 338卷 / 03期

关键词：

HIV-1; PROCESSING; GLYCOPROTEIN; ENDOPROTEASE; CALCIUM ION;

D O I：

10.1016/0014-5793(94)80284-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complete activation of human immunodeficiency virus type 1 (HIV-1) requires the endoproteolytic cleavage by cellular protease of the envelope glycoprotein precursor (gp160) into the external glycoprotein gp 120, and the transmembrane glycoprotein gp41. We report here the effect of depletion of cellular calcium ions on maturation of precursor gp 160 and its concomitant effect on syncytium formation. We show that the cellular endoprotease activity responsible for gp160 maturation and the capacity for HIV-1 to induce syncytium formation are calcium-dependent. In addition, we show that endoproteolytic maturation is a key step in syncytium formation induced by HIV-1.

引用

页码：281 / 284

页数：4

共 24 条

[1] MAMMALIAN SUBTILISINS - THE LONG-SOUGHT DIBASIC PROCESSING ENDOPROTEASES
BARR, PJ
[J]. CELL, 1991, 66 (01) : 1 - 3
[2] INFLUENCE OF CARBOHYDRATE MOIETIES ON THE IMMUNOGENICITY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RECOMBINANT GP160
BENJOUAD, A
GLUCKMAN, JC
ROCHAT, H
MONTAGNIER, L
BAHRAOUI, E
[J]. JOURNAL OF VIROLOGY, 1992, 66 (04) : 2473 - 2483
[3] MUTATIONAL ANALYSIS OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ENV GENE-PRODUCT PROTEOLYTIC CLEAVAGE SITE
BOSCH, V
PAWLITA, M
[J]. JOURNAL OF VIROLOGY, 1990, 64 (05) : 2337 - 2344
[4] THE CD4 (T4) ANTIGEN IS AN ESSENTIAL COMPONENT OF THE RECEPTOR FOR THE AIDS RETROVIRUS
DALGLEISH, AG
BEVERLEY, PCL
CLAPHAM, PR
CRAWFORD, DH
GREAVES, MF
WEISS, RA
[J]. NATURE, 1984, 312 (5996) : 763 - 767
[5] CALCIUM-IONS ARE REQUIRED FOR CELL-FUSION MEDIATED BY THE CD4-HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 ENVELOPE GLYCOPROTEIN INTERACTION
DIMITROV, DS
BRODER, CC
BERGER, EA
BLUMENTHAL, R
[J]. JOURNAL OF VIROLOGY, 1993, 67 (03) : 1647 - 1652
[6] ROLE OF N-LINKED GLYCANS OF ENVELOPE GLYCOPROTEINS IN INFECTIVITY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1
FENOUILLET, E
GLUCKMAN, JC
BAHRAOUI, E
[J]. JOURNAL OF VIROLOGY, 1990, 64 (06) : 2841 - 2848
[7] MAMMALIAN SUBTILISIN-RELATED PROTEINASES IN CLEAVAGE ACTIVATION OF THE PARAMYXOVIRUS FUSION GLYCOPROTEIN - SUPERIORITY OF FURIN PACE TO PC2 OR PC1/PC3
GOTOH, B
OHNISHI, Y
INOCENCIO, NM
ESAKI, E
NAKAYAMA, K
BARR, PJ
THOMAS, G
NAGAI, Y
[J]. JOURNAL OF VIROLOGY, 1992, 66 (11) : 6391 - 6397
[8] CHARACTERIZATION OF AN HIV-1 POINT MUTANT BLOCKED IN ENVELOPE GLYCOPROTEIN CLEAVAGE
GUO, HG
VERONESE, FD
TSCHACHLER, E
PAL, R
KALYANARAMAN, VS
GALLO, RC
REITZ, MS
[J]. VIROLOGY, 1990, 174 (01) : 217 - 224
[9] INHIBITION OF FURIN-MEDIATED CLEAVAGE ACTIVATION OF HIV-1 GLYCOPROTEIN-GP160
HALLENBERGER, S
BOSCH, V
ANGLIKER, H
SHAW, E
KLENK, HD
GARTEN, W
[J]. NATURE, 1992, 360 (6402) : 358 - 361
[10] HOSAKA M, 1991, J BIOL CHEM, V266, P12127

← 1 2 3 →