A NEW OLD YELLOW ENZYME OF SACCHAROMYCES-CEREVISIAE

被引:109
作者
NIINO, YS [1 ]
CHAKRABORTY, S [1 ]
BROWN, BJ [1 ]
MASSEY, V [1 ]
机构
[1] UNIV MICHIGAN,SCH MED,DEPT BIOL CHEM,ANN ARBOR,MI 48109
关键词
D O I
10.1074/jbc.270.5.1983
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In 1993, the first gene of Old Yellow Enzyme (OYE) of Saccharomyces cerevisiae was cloned (Stott, K,, Saito, K,, Thiele, D. J,, and Massey, V, (1993) J, Biol, Chem, 268, 6097-6106) and named OYE2 to distinguish it from the first OYE gene cloned from Saccharomyces carlsbergenesis (Saito, K,, Thiele, D, J,, Davio, M,, Lockridge, O., and Massey, V, (1991) J, Biol, Chem, 266, 20720-20724), The analysis of an OYE2 deletion mutant suggested that S, cerevisiae had at least two OYE genes. In the present study, we cloned a new OYE species named OYE3 and analyzed the OYE3 protein expressed in Escherichia coli, OYE3 consists of 400 amino acid residues and its molecular mass calculated by electrospray mass spectrometry is 44,788 daltons, in good agreement with the value of 44,920 daltons predicted from the amino acid sequence derived from the DNA sequence, In the downstream region of the OYE3 gene, the cytochrome oxidase (COX10) gene exists with a 426-base pair intermediate sequence, Some of the physicochemical and kinetic properties of OYE2 and OYE3 have been determined. Although the two enzymes are clearly closely related, they show differences in ligand binding properties and in their catalytic activities with oxygen and cyclohexen-2-one as accepters.
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页码:1983 / 1991
页数:9
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