LEUKEMIC TRANSFORMATION OF PRIMARY MYELOFIBROSIS - IMMUNOPHENOTYPE, GENOTYPE AND GROWTH-CHARACTERISTICS OF BLAST CELLS

被引：9

作者：

KIMURA, A

KAWAISHI, K

NAKATA, Y

HYODO, H

KURAMOTO, A

SATOW, Y

机构：

[1] Department of Environment and Mutation, Hiroshima University, Hiroshima

[2] Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima

来源：

LEUKEMIA & LYMPHOMA | 1995年 / 19卷 / 5-6期

关键词：

PRIMARY MYELOFIBROSIS; MYELOID LEUKEMIA; GM-CSF; IL-3; G-CSF; STEM CELL FACTOR;

D O I：

10.3109/10428199509112210

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Blast cells from six patients with leukemic transformation of primary myelofibrosis (PMF) were studied by morphology, immunophenotype and genotype as well as response to hematopoietic growth factors. The majority of the patients showed granulocytic or granulo-monocytic blasts, and only one had T lymphoid-monocytic blasts. None of the patients showed rearrangement of Ig or TCR genes, or the existence of the bcr-abl fused gene. A prominent growth response to GM-CSF and IL-3 was evident in all of the patients examined in liquid as well as semisolid cultures. The response to G-CSF was observed in four of the six patients in suspension culture, and in two of three patients in the clonogenic assay. Stem cell factor (SCF) was a weak growth stimulant, however the combination of this factor with GM-CSF or IL-3 was synergistically stimulatory. These results suggest that leukemic transformation of PMF occurs mainly at the level of myeloid stem cell, and that GM-CSF, IL-3, G-CSF and SCF are major growth factors for the blast cells in these cases.

引用

页码：493 / 498

页数：6

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