HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF 18 ALPHA-GLYCYRRHETINIC ACID AND 18 BETA-GLYCYRRHETINIC ACID IN RAT PLASMA - APPLICATION TO PHARMACOKINETIC STUDY

被引：31

作者：

TSAI, TH

CHEN, CF

机构：

[1] NATL YANG MING MED COLL,DEPT PHARMACOL,TAIPEI 11221,TAIWAN

[2] NATL RES INST CHINESE MED,TAIPEI,TAIWAN

[3] NATL YANG MING MED COLL,INST PHARMACOL,TAIPEI 11221,TAIWAN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 567卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80146-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the separation and determination of 18-alpha-glycyrrhetinic acid and 18-beta-glycyrrhetinic acid has been developed. Indomethancin was used as an internal standard. The drugs were separated on a reversed-phase column and detected by UV detection at a wavelength of 254 nm. Methanol-water-perchloric acid-ammonia (80:20:0.4:0.4, v/v) was used as the mobile phase at pH of 7.0-7.5. The detection limit of both compounds was 0.1-mu-g/ml in rat plasma. The method was applied to pharmacokinetic studies of glycyrrhetinic acids in rats. The results suggest that the pharmacokinetics appeared to be non-linear in nature.

引用

页码：405 / 414

页数：10

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