GOAT LIVER BETA-MANNOSIDASES - MOLECULAR-PROPERTIES, INHIBITION AND INACTIVATION OF THE LYSOSOMAL AND NONLYSOSOMAL FORMS

被引:31
作者
CAVANAGH, KT
FISHER, RA
LEGLER, G
HERRCHEN, M
JONES, MZ
JULICH, E
SEWELLALGER, RP
SINNOTT, ML
WILKINSON, FE
机构
[1] MICHIGAN STATE UNIV, DEPT PATHOL, E LANSING, MI 48824 USA
[2] MICHIGAN STATE UNIV, DEPT BIOCHEM, E LANSING, MI 48824 USA
[3] UNIV COLOGNE, INST BIOCHEM, D-5000 COLOGNE 41, FED REP GER
[4] UNIV BRISTOL, DEPT ORGAN CHEM, BRISTOL BS8 1TH, AVON, ENGLAND
关键词
D O I
10.1159/000469366
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The two caprine hepatic .beta.-mannosidases have been partially purified and their properties have been compared. The lysosomal .beta.-mannosidase A had an apparent molecular weight of 127,000 .+-. 10,000 and an isoelectric point of pH 6-7. Its activity was unaffected by incubation with Triton X-100 (0.1%) and cysteine (20 mM) and it hydrolyzed the presumed natural substrates, Man(.beta.1-4)GlcNAc and Man(.beta.1-4)GlcNaC(.beta.1-4)GlcNAc. The nonlysosomal .beta.-mannosidase B had an apparent molecular weight of 43,000 .+-. 2,000 and an isoelectric point of pH 5.5. .beta.-Mannosidase B was activated by Triton X-100 (0.1%) and was inhibited by cysteine (20 mM). Hydrolysis of Man(.beta.1-4)GlcNAc, but not of Man(.beta.1-4)GlcNAc(.beta.1-4)GlcNAc, followed incubation with .beta.-mannosidase B. 1,5-Dideoxy-1,5-imino-D-mannitol did not inhibit the A enzyme and only feebly (Ki = 0.3 mM) inhibited the B enzyme; .beta.-D-mannopyranosylmethyl p-nitrophenyl triazene did not inactivate either enzyme but 1,2-anhydro-1,2,3,5,6/4-cyclohexane hexol inactivated the B enzyme only. The radical mechanistic differences between the two enzymes argue against their having the same genetic origin.
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页码:75 / 82
页数:8
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