TRANSGLUTAMINASE IN RESPONSE TO HYPERTONIC NACL-INDUCED GASTRIC-MUCOSAL INJURY IN RATS

Background: Polyamines serve as substitutes for transglutaminase-catalyzed protein cross-linking and are essential to the healing of gastric mucosal lesions. This study determines whether transglutaminase and protein cross-linking have a role in the healing of hypertonic NaCl-induced gastric lesions. Methods: Rats were fasted 22 hours before given 1 ml 3.4 Mol/L NaCl intragastrically. Gastric mucosa was examined histologically and grossly, and transglutaminase activity was measured as the Ca2+-dependent covalent incorporation of [3H]putrescine into acid-precipitable protein. Results: Transglutaminase activity increased significantly from 2 to 8 hours, peaking between 4 and 6 hours after NaCl administration. Lesions were significantly produced after 2 hours, and damage paralleled transglutaminase activity. Dansylcadaverine (200 mg/kg orally), a specific inhibitor of protein cross-linking, prevented the increases in transglutaminase activity and significantly delayed healing but had no effect on lesion formation. Conclusions: These results indicate that (1) hypertonic NaCl-induced gastric mucosal damage is associated with a significant increase in transglutaminase activity and (2) increased transglutaminase activity is involved in the mechanism of normal mucosal healing. © 1993.