RESOLVING GENETIC-RELATIONSHIPS WITH MICROSATELLITE MARKERS - A PARENTAGE TESTING SYSTEM FOR THE SWALLOW HIRUNDO-RUSTICA

被引：183

作者：

PRIMMER, CR

MOLLER, AP

ELLEGREN, H

机构：

[1] SWEDISH UNIV AGR SCI,UPPSALA BIOMED CTR,DEPT ANIM BREEDING & GENET,S-75124 UPPSALA,SWEDEN

[2] UNIV COPENHAGEN,INST ZOOL,DK-2100 COPENHAGEN,DENMARK

来源：

MOLECULAR ECOLOGY | 1995年 / 4卷 / 04期

关键词：

DNA FINGERPRINTING; EXTRA-PAIR PATERNITY; LINKAGE ANALYSIS; MICROSATELLITES; MUTATION RATE; NULL ALLELE; PARENTAGE TESTING;

D O I：

10.1111/j.1365-294X.1995.tb00243.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eight polymorphic microsatellite markers from the swallow were isolated and characterized. Extraordinary variability was revealed at the HrU6 locus with 45 different alleles scored among 46 unrelated individuals. The probability that the same genotype combination would occur in two random and unrelated individuals at six selected loci was as low as 1.3 x 10(-8) and the combined exclusion probability was 0.9996. Stable Mendelian inheritance was observed in about 1000 meioses. No significant linkage was revealed and for almost all combinations of marker-pairs, linkage closer than 5 cM could be excluded. At two loci, null (nonamplifying) alleles were encountered. Thirteen (30%) extra-pair offspring were identified in 5 (56%) broods when applying the marker set on a nearly complete swallow colony. We were able to identify a single male from the or-her families in the colony as the most likely father for nine of the 13 extra-pair offspring.

引用

页码：493 / 498

页数：6

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