PRODUCTION OF MONOCLONAL-ANTIBODIES BY HYBRIDOMA CELLS IN A FLAT SHEET MEMBRANE BIOREACTOR

被引：9

作者：

HAGEDORN, J ^{[1
]}

KARGI, F ^{[1
]}

机构：

[1] WASHINGTON UNIV,DEPT CHEM ENGN,BIOTECHNOL ENGN LAB,ST LOUIS,MO 63130

来源：

BIOTECHNOLOGY PROGRESS | 1990年 / 6卷 / 03期

关键词：

D O I：

10.1021/bp00003a011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The purpose of this study was to investigate hybridoma growth and monoclonal antibody formation in a flat sheet membrane bioreactor. The effects of several different molecular weight cutoff membranes on growth and antibody formation were investigated. Nutrient and toxic product diffusion through the membranes were quantified, and the kinetic and physical constants of the system were determined. Copyright © 1990 American Institute of Chemical Engineers (AIChE)

引用

页码：220 / 224

页数：5

共 9 条

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[2]

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[3]

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[4]

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[5]

Lavery M., Dearns M.J., Price D.J., Emery A.N., Physical Conditions During Batch Culture of Hybridomas in Laboratory Scale Stirred Tank Reactors, Dev. Biol. Stand., 60, pp. 199-206, (1985)

[6]

Sever S., Commercial Production of Monoclonal Antibodies, pp. 49-71, (1987)

[7]

Sigma Glucose Diagnostic Kit, Procedure 115, (1988)

[8]

Sigma Pyruvate/Lactate Diagnostic Kit, Procedure 726, (1988)

[9]

Sigma Ammonia Diagnostic Kit, Procedure 170‐UV, (1988)

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