PURIFICATION AND CHARACTERIZATION OF IMMUNOGLOBULIN PRODUCTION STIMULATING FACTOR DERIVED FROM HUMAN-B LYMPHOBLASTOID HO-323 CELLS

被引：18

作者：

TOYODA, K ^{[1
]}

SUGAHARA, T ^{[1
]}

INOUYE, K ^{[1
]}

YAMADA, K ^{[1
]}

SHIRAHATA, S ^{[1
]}

MURAKAMI, H ^{[1
]}

机构：

[1] KYUSHU UNIV 4609,FAC AGR,GRAD SCH GENET RESOURCES TECHNOL,6-10-1 HAKOZAKI,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

CYTOTECHNOLOGY | 1990年 / 3卷 / 02期

关键词：

human B lymphoblastoid cell line HO-323; hybridoma; immunoglobulin production stimulating factor; serum-free culture;

D O I：

10.1007/BF00143681

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An immunoglobulin (Ig) production stimulating factor (IPSF) for hybridomas was found in spent medium of the human B lymphoblastoid cell line, HO-323. The IPSF was purified by serial use of DEAE chromatography, ultrafiltration, gel filtration and HPLC-DEAE chromatography. Purified IPSF was estimated to be a 410 k macro molecule by gel filtration, and contained three types of isomers which were separated from each other by native polyacrylamide gel electrophoresis. All of the isomers were, however, assumed to have the same protein components by SDS polyacrylamide gel electrophoresis. The IPSF was effective for human-human and mouse-mouse hybridomas producing IgM, but not for IgG producers in the experimental condition used here. Human-human hybridoma HF10B4, cultured in IPSF-containing medium, produced 20 times more IgM than in IPSF-free medium under serum-free conditions. The IPSF showed very little proliferation stimulating activity on HF10B4 cells. © 1990 Kluwer Academic Publishers.

引用

页码：189 / 197

页数：9

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