A SINGLE CELL OBSERVATION OF STAUROSPORINE EFFECT ON THE CA-2+ SIGNALS IN RAT BASOPHILIC LEUKEMIA-CELLS

被引：25

作者：

TESHIMA, R

IKEBUCHI, H

TERAO, T

MIYAGAWA, T

ARATA, Y

NAKANISHI, M

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,BUNKYO KU,TOKYO 113,JAPAN

[2] NAGOYA CITY UNIV,FAC PHARMACEUT SCI,MIZUHO KU,NAGOYA,AICHI 467,JAPAN

来源：

FEBS LETTERS | 1990年 / 270卷 / 1-2期

关键词：

Calcium signal; Microscopic image analysis; RBL-2H3; Staurosporine;

D O I：

10.1016/0014-5793(90)81247-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A digital imaging fluorescence microscope was used to study the effect of a protein kinase inhibitor staurosporine on the antigen-dependent calcium signals in an individual rat basophilic leukemia cell (RBL-2H3). Although dose dependency of staurosporine was different from one cell to another, staurosporine inhibited, at low concentration, the calcium influx from the external medium into RBL-2H3 cells. At high concentration, however, it inhibited both the removal of calcium ion from internal stores and the calcium influx from the external medium. These results indicated that staurosporine is necessary for the inhibition of the calcium influx from the external medium and that a protein kinase (possibly protein kinase C) is involved in the calcium influx from the external medium into the cytoplasm. © 1990.

引用

页码：115 / 118

页数：4

共 12 条

[1]

BARUSUMIAN EL, 1981, EUR J IMMUNOL, V11, P317

[2]

BEAVEN MA, 1984, J BIOL CHEM, V259, P7129

[3] PRODUCTION OF MONOCLONAL MOUSE IGE ANTIBODIES WITH DNP SPECIFICITY BY HYBRID CELL-LINES [J].