NOVEL REGULATORY ROLE OF PHOSPHORYLATED CLATHRIN LIGHT CHAIN-BETA IN BOVINE BRAIN COATED VESICLES

被引：5

作者：

HANSON, VG ^{[1
]}

SCHOOK, WJ ^{[1
]}

PUSZKIN, S ^{[1
]}

机构：

[1] CUNY MT SINAI SCH MED,DEPT PATHOL,CELLULAR PATHOL LAB,NEW YORK,NY 10029

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 01期

关键词：

50‐Kilodalton polypeptide; Clathrin light chain β; Clathrin‐coated vesicles; Mg[!sup]2+[!/sup]‐ATP‐dependent phosphatase;

D O I：

10.1111/j.1471-4159.1990.tb13281.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: The 50‐kilodalton (kDa) assembly polypeptide of bovine brain clathrin coated vesicles (CCVs) is phosphorylated in a cyclic nucleotide‐ and Ca2+‐independent manner and is dephosphorylated by a Mg2+‐ATP‐dependent CCV phosphatase. This report provides evidence for modulation of the phosphorylation reaction of the 50‐kDa assembly polypeptide by phosphorylated clathrin light chain β (pLCβ). In vitro, phosphorylated LCβ inhibits phosphorylation of the 50‐kDa polypeptide in CCVs. Furthermore, incubation of previously phosphorylated 50‐kDa polypeptide in CCVs with phosphorylated LCβ results in a rapid dephosphorylation of the 50‐kDa assembly polypeptide. Both phenomena are time and concentration dependent. Monoclonal antibodies to LCβ prevent the modulatory effect of phosphorylated LCβ on the 50‐kDa assembly polypeptide phosphorylation in CCVs. The results obtained indicate for the first time, to our knowledge, that phosphorylated LCβ has a modulatory role in CCVs. The data also suggest that phosphorylated LCβ promotes activation of a coated vesicle phosphatase. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：46 / 50

页数：5

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