FORMATION OF STABLE P53 HOMOTETRAMERS AND MULTIPLES OF TETRAMERS

被引：116

作者：

STENGER, JE ^{[1
]}

MAYR, GA ^{[1
]}

MANN, K ^{[1
]}

TEGTMEYER, P ^{[1
]}

机构：

[1] SUNY STONY BROOK,DEPT MICROBIOL,STONY BROOK,NY 11794

来源：

MOLECULAR CARCINOGENESIS | 1992年 / 5卷 / 02期

关键词：

OLIGOMERIZATION; QUATERNARY STRUCTURE; TUMOR SUPPRESSOR;

D O I：

10.1002/mc.2940050204

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used gradient gel electrophoresis and chemical cross-linking to analyze the quaternary structure of purified, wild-type, murine p53. Under nondenaturing conditions, p53 electrophoreses as tetramers and multiples of tetramers. Under denaturing conditions, fully cross-linked p53 also behaves as tetrameric structures. We confirmed the composition of the tetramers by partially cross-linking p53 and dissociating tetramers into monomers, dimers, and trimers.

引用

页码：102 / 106

页数：5

共 34 条

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