QUANTITATIVE X-RAY MAPPING OF BIOLOGICAL CRYOSECTIONS

被引：32

作者：

FIORI, CE ^{[1
]}

LEAPMAN, RD ^{[1
]}

SWYT, CR ^{[1
]}

ANDREWS, SB ^{[1
]}

机构：

[1] NINCDS, NEUROBIOL LAB, BETHESDA, MD 20892 USA

来源：

ULTRAMICROSCOPY | 1988年 / 24卷 / 2-3期

关键词：

IMAGING TECHNIQUES - X-Ray - SIGNAL PROCESSING - Digital Techniques;

D O I：

10.1016/0304-3991(88)90313-0

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The potential for applying X-ray mapping to the elemental microanalysis of biological cryosections is discussed. Methods are described for acquiring and processing data, including use of the top-hat digital filter to remove the average effects of the background contribution. Practical considerations for X-ray mapping are discussed in terms of typical counts per pixel and minimum detectability which depends on the number of pixels chosen to integrate the signal. These aspects are illustrated with elemental maps (Na, P, K, Ca and Fe) from freeze-dried cryosections of mouse cerebellar cortex. A calcium sensitivity in the range 0. 5 to 2. 5 mmol/kg wet weight of tissue is demonstrated.

引用

页码：237 / 249

页数：13

共 40 条

[1]

ALTMAN PL, 1974, BIOL DATA BOOK, V3, P1771

[2] INTRACELLULAR STRUCTURE AND ELEMENTAL ANALYSIS IN RAPID-FROZEN NEURONS [J].