ISOLATION AND CHARACTERIZATION OF A NEW STRAIN OF CELLULOMONAS-FLAVIGENA

被引:6
作者
ANGELO, R [1 ]
VOEPEL, KC [1 ]
BULLER, CS [1 ]
机构
[1] UNIV KANSAS,DEPT MICROBIOL,LAWRENCE,KS 66045
来源
JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1990年 / 5卷 / 2-3期
关键词
Cellulomonas flavigena; Intracellular inclusion; Morphology;
D O I
10.1007/BF01573861
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A cellulolytic bacterium was isolated from leaf litter. Its nutritional characteristics and most of its morphological features closely resemble those of ATCC # 482, which is considered to be the type species of Cellulomonas flavigena (Stackebrandt and Keddie in Bergey's Manual of Systematic Bacteriology). However, when stationary phase cells from cultures using a minimal medium containing an excess carbon and energy source are compared, a very prominent morphological difference is manifest. Phase contrast microscopy and transmission electron microscopy indicate the presence of large swollen polar structures in the type species. The absence of such structures from the new isolate and the production of large amounts of an extracellular polysaccharide indicate that it is a new strain of Cellulomonas flavigena. © 1990 Society for Industrial Microbiology.
引用
收藏
页码:125 / 129
页数:5
相关论文
共 11 条
[1]  
Buller C.S., Voepel K.C., Production and purification of an extracellular polyglucan produced by Cellulomonas flavigena strain KU, J. Ind. Microbiol., 5, pp. 139-146, (1990)
[2]  
Clarke F.E., Criteria suitable for species differentiation in Cellulomonas and a revision of the genus, Int. Bull. Bacteriol. Nomencl. Taxon., 3, pp. 179-199, (1953)
[3]  
Keddie R.M., Cellulomonas, Bergey's Manual of Determinative Bacteriology, pp. 629-631, (1974)
[4]  
Keddie R.M., Leask B.G.S., Grainger J.M., A comparison of coryneform bacteria from soil and herbage. Cell wall compositon and nutrition, Journal of Applied Bacteriology, 29, pp. 17-43, (1966)
[5]  
Kim B.H., Wimpenny J.W.T., Growth and cellulolytic activity of Cellulomonas flavigena, Can. J. Microbiol., 27, pp. 1260-1266, (1981)
[6]  
Luft J.H., Improvements in epoxy resin embedding methods, J. Biophys. Biochem. Cytol., 9, pp. 409-414, (1961)
[7]  
Reynolds E.S., The use of lead citrate at high pH as an electron-opaque stain in electron microscopy, J. Cell Biol., 17, pp. 208-213, (1963)
[8]  
Ryter A., Kellenberger E., Etude an microscope electronique de plasmas contenant de l'acide desoxyribonucleique, Z. Naturforsch., 13, pp. 597-605, (1958)
[9]  
Staley J.T., Krieg N.R., Classification of procaryotic organisms: an overview, Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1-4, (1986)
[10]  
Stackebrandt E., Keddie R.M., Cellulomonas, Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1325-1329, (1986)