THE PURIFICATION AND CHARACTERIZATION OF CERVINE IGM AND IGG

被引：4

作者：

HIBMA, MH ^{[1
]}

GRIFFIN, JFT ^{[1
]}

机构：

[1] UNIV OTAGO, DEPT MICROBIOL, DUNEDIN, NEW ZEALAND

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1990年 / 26卷 / 04期

关键词：

D O I：

10.1016/0165-2427(90)90118-C

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A procedure is described for the isolation of immunoglobulin G (IgG) and immunoglobulin M (IgM) from hyperimmune cervine serum. Hybrids of red deer (Cervus elaphus) and wapiti (Cervus canadensis) were immunised with keyhole limpet hemocyanin (KLH). An immunoglobulin-containing fraction was precipitated from the hyperimmune serum using ammonium sulphate. The antigen-specific immunoglobulins were purified by KLH-conjugated sepharose affinity chromatography and further separated into IgM and IgG by gel-filtration chromatography. Purified immunoglobulin was analysed by polyacrylamide gel electrophoresis and isoelectric focusing. The molecular weights and isoelectric points of the composite chains of cervine IgG and IgM are presented.

引用

页码：343 / 352

页数：10

共 15 条

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